Biotin-11-UTP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shelf Life: 12 months after date of delivery

Exact Mass: 878.17 g/mol (free acid)

Form: filtered solution (30 kDa) in 10 mM Tris-HCl

Color: colorless to slightly yellow

Ph: 7.5 ±0.5

Spectroscopic Properties: λ_max 289 nm, ε 7.1 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:  
Microarray based hybridization detection^{[1, 2]}
RNA-labeling^[2]
Biotin-PAGE^[3]
ISH^[4] 
 [1] Lonergan et al. (2007) Comparison of target labeling methods for use of Affymetrix GeneChips. BMC Biotechnology 7:24.
[2] Glaid et al. (1989) Non-isotopic RNA probes. Comparison between different labels and detection systems. Histochemistry 93:91.
[3] Theissen et al. (1989) Degree of biotinylation in nucleic acids estimated by a gel retardation assay. Analyt. Biochem. 179:98.
[4] Baumann et al. (1988) Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization. Cytometry 9:517.

Formula: C₂₈H₄₅N₆O₁₈P₃S (free acid)

Molecular Weight: 878.67 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Concentration: 10 mM - 11 mM

Storage: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shipping Condition: shipped on gel packs